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Journal: Lupus science & medicine
Article Title: Proteomics uncovers ICAM2 (CD102) as a novel serum biomarker of proliferative lupus nephritis.
doi: 10.1136/lupus-2024-001446
Figure Lengend Snippet: Figure 2 ELISA validation of three serum biomarkers using an independent cohort and their correlation with clinical indices. (A–C) A cohort of 80 serum samples: 30 healthy controls (HC, green) and 50 patients with lupus nephritis (LN, red) were tested by ELISA for the levels of ICAM2, TIMP1 and THBS1. (D) The discriminatory abilities of the three serum markers were assessed in distinguishing patients with LN from HCs using receiver operating characteristic (ROC) curve analysis. (E–K) The correlation of ELISA-assayed serum ICAM2 levels with clinical indices in LN. r denotes Spearman’s correlation coefficient. ****p<0.0001. AUC, area under the curve.
Article Snippet:
Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery
Journal: International Journal of Medical Sciences
Article Title: Establishment of a nomogram model based on immune-related genes using machine learning for aortic dissection diagnosis and immunomodulation assessment
doi: 10.7150/ijms.100572
Figure Lengend Snippet: Validation of the four hub genes expression in control individuals and AD patients. ( A - B ). Protein level for CXCL1, TIMP1, ITGA5 and PTX3 in healthy donor (D1-D10) and AD patients (D1-D10) aortic samples. β-actin and Vinculin were used as internal control. *** P <0.001. ( C ) mRNA expression level of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). β-actin was used as internal control. ( D ) Serum concentration of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). * P < 0.05, ** P < 0.01, *** P <0.001.
Article Snippet: CXCL1 (Elab Sciences, #E-EL-H0045),
Techniques: Biomarker Discovery, Expressing, Control, Concentration Assay
Journal: International Journal of Medical Sciences
Article Title: Establishment of a nomogram model based on immune-related genes using machine learning for aortic dissection diagnosis and immunomodulation assessment
doi: 10.7150/ijms.100572
Figure Lengend Snippet: Validation of the four hub genes expression in control individuals and AD patients. ( A - B ). Protein level for CXCL1, TIMP1, ITGA5 and PTX3 in healthy donor (D1-D10) and AD patients (D1-D10) aortic samples. β-actin and Vinculin were used as internal control. *** P <0.001. ( C ) mRNA expression level of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). β-actin was used as internal control. ( D ) Serum concentration of CXCL1, ITGA5, PTX3 and TIMP1 in healthy donors (D1-D10) and AD patients (A1-A12). * P < 0.05, ** P < 0.01, *** P <0.001.
Article Snippet:
Techniques: Biomarker Discovery, Expressing, Control, Concentration Assay